Journal: Toxins

Article Title: Cytotoxic Effects and Intracellular Localization of Bin Toxin from Lysinibacillus sphaericus in Human Liver Cancer Cell Line

doi: 10.3390/toxins13040288

Figure Lengend Snippet: Morphology of Hs68 and HepG2 cells treated with mock control (MC; 1× PBS buffer) and protein toxins. Cells were treated with PS2 (0.5 µg/mL) and Bin proteins (50 µg/mL) for 24 h and then viewed by inverted light microscope (magnified 10×).

Article Snippet: Cell morphologies were also observed after toxin treatments under an inverted light microscope (Nikon Eclipse TS100, Melville, NY, USA) with a 10× objective lens at 24 h after inoculation.

Techniques: Control, Light Microscopy

Journal: Toxins

Article Title: Cytotoxic Effects and Intracellular Localization of Bin Toxin from Lysinibacillus sphaericus in Human Liver Cancer Cell Line

doi: 10.3390/toxins13040288

Figure Lengend Snippet: Fluorescence confocal microscopy of HepG2 cells treated with Oregon Green labeled BinA/Texas Red labeled BinB mixture. HepG2 cells were incubated with 25 and 50 μg/mL of Oregon Green labeled BinA/Texas Red labeled BinB mixture for 2 and 3 h to observe the internalization pattern in the cells. The nuclei were stained with DAPI. Three-dimensional views of Oregon Green labeled BinA/Texas Red labeled BinB treated cells were constructed from the selected depth two-dimensional images. Approximately 30 cells for each condition were analyzed. The localization of Oregon Green labeled BinA and Texas Red labeled BinB was observed using laser scanning confocal microscope, and images were processed from a three-dimensional view using Zen blue software.

Article Snippet: Cell morphologies were also observed after toxin treatments under an inverted light microscope (Nikon Eclipse TS100, Melville, NY, USA) with a 10× objective lens at 24 h after inoculation.

Techniques: Fluorescence, Confocal Microscopy, Labeling, Incubation, Staining, Construct, Microscopy, Software

Journal: Scientific Reports

Article Title: Synthesis and structure of a new thiazoline-based palladium(II) complex that promotes cytotoxicity and apoptosis of human promyelocytic leukemia HL-60 cells

doi: 10.1038/s41598-020-73488-0

Figure Lengend Snippet: Effects of chemotherapeutics on apoptosis in HL-60 cells. Cells were treated with 11.3 µM cisplatin, 20.7 µM PyTT, 20.7 µM PdPyTT, or the vehicle (DMSO, control) for 24 h. ( A ) Hoechst 33342-stained cells were visualized with epifluorescence microscopy and representative images of each experimental condition are shown. The fraction of apoptotic nuclei was evaluated as indicated in the Materials and Methods section. Yellow arrows indicate apoptotic nuclei (i.e., fragmented or condensed). Both fragmented and condensed nuclei are also shown in greater detail in the magnifications. ( B ) Histograms show percentages of cells with apoptotic nucleus. Values represent means ± S.D. of 6 independent experiments. * P < 0.05 compared to control values. Scale bar: 100 µm.

Article Snippet: Then, at least 100 cells per field were counted at the epifluorescence microscope (Nikon Eclipse TS100 equipped with a LED illumination system CoolLED pE-300 white and a digital camera DS-Qi1Mc) in at least three randomly selected microscopic fields.

Techniques: Control, Staining, Epifluorescence Microscopy